Figure 3.

Colony-blotting analysis of CPY and BiP missecretion. Integrants of wild-type ARF1 (NYY0:0) and arf1 ts mutant alleles (arf1-11, -12, -13, -14, -15, -16, -17, and -18) were grown on YPD for 1.5 d. Prewetted nitrocellulose filters were overlaid onto the streaks of the cells, incubated at a semipermissive temperature (35°C) for 20 h, and let react with anti-CPY or anti-BiP antisera to reveal secreted CPY (A) and BiP (B). Δvps1 (KUY165), Δslp1 (YW10–2B), and rer2 (SNH23-7D) were used as positive controls.