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Electron microscopic observation of the arf1-13 ts mutant. arf1-13 ts mutant cells were grown to an early log phase at 23°C, further incubated at 23°C (A) or at 37°C for 1 h (B and C) and 4 h (D, E, and F), and then subjected to freeze-fixation electron microscopy. In A, B and D, ring structures are indicated by arrows. Arrowheads in B indicate MVB-like structures. The vesicle marked with a double arrowhead harbors a double membrane. Bars, 1.0 μm.
