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Localization of GFP-Rer1p in arf1 ts mutants. Wild-type (YPH499, WT) and the indicated arf1 ts mutant cells were grown to an early log phase at 23°C, and then half of the culture was shifted to 37°C and incubated for 30 min. Images were visualized by fluorescence confocal (left) and Nomarski (right) microscopy. Bar, 5 μm.
