Table 2.
The newly obtained eight arf1 ts mutant alleles: mutation points in the amino acid sequence and growth phenotypes
| Mutation points | Growth
|
||||||
|---|---|---|---|---|---|---|---|
| 15°C | 23°C | 26.5°C | 30°C | 35°C | 37°C | ||
| Wild type | +++ | +++ | +++ | +++ | +++ | +++ | |
| Δarf2 | +++ | +++ | +++ | +++ | +++ | +++ | |
| arf1-11 | K38T E132D L173S | +++ | +++ | +++ | +/− | − | − |
| arf1-12 | K38T | +++ | +++ | +++ | +++ | +++ | +/− |
| arf1-13 | L173S | +++ | +++ | +++ | +++ | +++ | +/− |
| arf1-14 | F51I | +++ | +++ | +++ | +++ | + | − |
| arf1-15 | L25P | +++ | +++ | +++ | +++ | +++ | +/− |
| arf1-16 | E41V D129E | ++ | ++ | ++ | +/− | − | − |
| arf1-17 | E41V | +++ | +++ | +++ | +++ | +++ | − |
| arf1-18 | H80P | +++ | +++ | +++ | +++ | +++ | +/− |
Growth was examined on YPD plates. +++, wild-type growth; ++, growth slower than wild type; +, very slow growth (minimal single-colony formation); +/−, almost no growth (no single-colony formation); −, completely no growth.