Table 3.
Intragenic complementation between ts arf1 mutants
| 0α | 11α | 13α | 14α | 16α | 17α | 18α | |
|---|---|---|---|---|---|---|---|
| 0a | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
| 11a | +++ | − | − | − | +++a | + | ++ |
| 13a | +++ | − | − | − | − | + | +++ |
| 14a | +++ | − | − | − | + | +++ | +++ |
| 16a | +++ | +++a | − | + | − | − | +++ |
| 17a | +++ | + | + | +++ | − | − | +++ |
| 18a | +++ | ++ | +++ | +++ | +++ | +++ | − |
MATa and MATα cells of wild-type ARF1 (0) and arf1 ts mutants (arf1-11, arf1-13, arf1-14, arf1-16, arf1-17, and arf1-18) were crossed in all combinations. Diploids were selected and grown on YPD plates at 37°C for 4 d except for arf1-11 × arf1-16 (
a
, 30°C). +++, wild-type growth; ++, growth slower than wild type; +, very slow growth (minimal single-colony formation); −, completely no growth.