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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Nat Med. 2010 Nov 14;16(12):1444–1449. doi: 10.1038/nm.2260

Figure 4. Two–photon excitation of 6–week–old Abca4−/−Rdh8−/− (dko) intact mouse eye.

Figure 4

(a) Fluorescence intensity as a function of excitation wavelength, normalized to maximum emission at 720 nm excitation. (b) Emission spectra from RPE of WT and dko mice excited with laser light at 730 nm and 910 nm. Blue arrow points to 580 nm. (C) Images of dko mouse RPE at different excitation wavelengths. All images were obtained with 10 mW of laser power; PMT gain and offset were kept constant. Scale bar 50 μm. (d) Zoomed–in TPM image of unstained dko mouse RPE. Fluorescent granules are distributed uniformly throughout cells with clearly visible boundaries. Green color was arbitrarily chosen to make the image details more visible. Scale bar 25 μm.