Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2000 Nov 1;19(21):5835–5844. doi: 10.1093/emboj/19.21.5835

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2000 European Molecular Biology Organization

PMC Copyright notice

graphic file with name cdd563f3a.jpg — Fig. 3. Monolayers of Srf^–/–ES cells activate mesodermal marker genes when ectopically expressing SRF or when treated with RA. (A) Gene expression patterns in ES cells of different Srf genotype, including Srf ‘rescued’ cells. ES lines used were 226-100-2 (Srf^{–/–rescue}; lane 1), 226-100-37 (Srf^{–/–rescue}; lane 2), 226-100-42 (Srf^{–/–rescue}; lane 3), 226-100 (Srf^–/–; lane 4) and 226 (Srf^–/+; lanes 5 and 6). LIF removal was only done with the cells investigated in lane 6. Analysis was performed as described in the legend to Figure 2A. Lane 7 is a negative RT–PCR control lacking cDNA in the reaction. Of the three Srf^{–/–rescue} ES lines, 226-100-2 expressed the highest levels of SRF (approximately four times the endogenous levels). (B) Expression of differentiation markers in monolayers of ES cells of different Srf genotype upon induction of in vitro differentiation by LIF withdrawal plus simultaneous addition of RA. ES lines used were 226 (Srf^–/+; lanes 1–7) and 226-81 (Srf^–/–; lanes 8–14). Analysis was performed as described in the legend to Figure 2A.

graphic file with name cdd563f3b.jpg — Fig. 3. Monolayers of Srf^–/–ES cells activate mesodermal marker genes when ectopically expressing SRF or when treated with RA. (A) Gene expression patterns in ES cells of different Srf genotype, including Srf ‘rescued’ cells. ES lines used were 226-100-2 (Srf^{–/–rescue}; lane 1), 226-100-37 (Srf^{–/–rescue}; lane 2), 226-100-42 (Srf^{–/–rescue}; lane 3), 226-100 (Srf^–/–; lane 4) and 226 (Srf^–/+; lanes 5 and 6). LIF removal was only done with the cells investigated in lane 6. Analysis was performed as described in the legend to Figure 2A. Lane 7 is a negative RT–PCR control lacking cDNA in the reaction. Of the three Srf^{–/–rescue} ES lines, 226-100-2 expressed the highest levels of SRF (approximately four times the endogenous levels). (B) Expression of differentiation markers in monolayers of ES cells of different Srf genotype upon induction of in vitro differentiation by LIF withdrawal plus simultaneous addition of RA. ES lines used were 226 (Srf^–/+; lanes 1–7) and 226-81 (Srf^–/–; lanes 8–14). Analysis was performed as described in the legend to Figure 2A.