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. 2000 Nov 1;19(21):5835–5844. doi: 10.1093/emboj/19.21.5835

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graphic file with name cdd563f6a.jpg — Fig. 6. Expression of differentiation marker genes in EBs generated from ES cells of different Srf genotype. (A) Semiquantitative RT–PCR. EBs, derived from the indicated ES lines (99 Srf^–/+, 81 Srf^–/– and 100 Srf^–/–) were differentiated for 2 days (lanes 2, 5 and 8), 5 days (lanes 3, 6 and 9) and 8 days (lanes 4, 7 and 10). Lane 1 provides size markers and subjected to RT–PCR analysis. The marker genes investigated were: keratin 18 (a marker of endoderm and trophectoderm), Bmp2 (endoderm, mesoderm), Bmp4 (ectoderm, mesoderm), T(Bra) (mesoderm), vimentin (mesoderm, parietal endoderm) and α-fetoprotein (visceral endoderm). (B) Quantitative RT–PCR. The indicated marker genes, Bmp2, Bmp4 and T(Bra), were analyzed in EBs identical to those described in (A).

graphic file with name cdd563f6b.jpg — Fig. 6. Expression of differentiation marker genes in EBs generated from ES cells of different Srf genotype. (A) Semiquantitative RT–PCR. EBs, derived from the indicated ES lines (99 Srf^–/+, 81 Srf^–/– and 100 Srf^–/–) were differentiated for 2 days (lanes 2, 5 and 8), 5 days (lanes 3, 6 and 9) and 8 days (lanes 4, 7 and 10). Lane 1 provides size markers and subjected to RT–PCR analysis. The marker genes investigated were: keratin 18 (a marker of endoderm and trophectoderm), Bmp2 (endoderm, mesoderm), Bmp4 (ectoderm, mesoderm), T(Bra) (mesoderm), vimentin (mesoderm, parietal endoderm) and α-fetoprotein (visceral endoderm). (B) Quantitative RT–PCR. The indicated marker genes, Bmp2, Bmp4 and T(Bra), were analyzed in EBs identical to those described in (A).