Fig. 4. GFP–LC3 does not co-localize with a lysosomal protein lamp1 in bafilomycin A₁-treated cells under starvation conditions. HeLa cells transiently transfected with GFP–LC3 were cultured at 37°C for 90 min in Hanks’ solution containing 0.1 µM bafilomycin A₁ and analysed by immunofluorescence confocal microscopy using antibody against lamp1 and rhodamine-conjugated second antibody. (A) GFP–LC3 labelling, (B) lamp1 staining and (C) a merged image of the same field are shown. Bar, 10 µm.