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Slices of the 3D NMR spectra collected on calcium-saturated 13C,15N-CaM1-148 in complex with the unlabeled βCaNp. (A and D) slices from 13C-edited and 12C,14N-filtered 3D NOESY spectrum. (B and E) slices from 3D HCCH-TOCSY spectrum. (C and F) slices from 13C-edited 3D NOESY spectrum. Panels A-C were taken at the Cα of P43, while panels D-F were taken at the Cα of P43. The 1H assignments are indicated at the top. Intermolecular NOEs are labeled.
