TABLE 3.

Free Energies of Calcium Binding to CaM In the Presence of βCaNp

	βCaNp	aΔG1app	aΔG2app	ΔΔG2b
Sites I & II
CaM1-80	−	−6.02 ± 0.09	−12.16 ± 0.07	−0.39
	+	−6.34 ± 0.23	−12.55 ± 0.29
CaM1-148	−	−6.35 ± 0.25	−12.21 ± 0.06	−6.09
	+	−8.68 (fixed)	−18.30 ± 0.39
Sites III & IV
CaM76-148	−	−7.32 ± 0.20	−15.71 ± 0.05	−2.62
	+	−9.21 ± 0.06	−18.33 ± 0.19
CaM1-148	−	−7.25 ± 0.08	−16.03 ± 0.04	−4.75
	+	−10.09 ± 0.70	−20.78 ± 0.27

^aGibbs free energies (in kcal/mol (1 kcal = 4.184 kJ)) are described. Reported free energies and errors represent averages and standard deviations for three or more trials.

^bΔΔG₂ (kcal/mol) = ΔG_{2 (βCaNp)} - ΔG_{2 (no βCaNp)}