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. Author manuscript; available in PMC: 2012 Mar 15.
Published in final edited form as: Biochemistry. 2011 Feb 8;50(10):1634–1640. doi: 10.1021/bi101988s

Figure 3. Labeling of given single-Cys replacements in helix VII or helix X.

Figure 3

Single-Cys mutants Q241C, A244C, S311C, A312C, L313C, L329C, L330C, V331C, F334C and T338C were labeled with TMRM for 30 s at 0 °C. TMRM-labeled bands (upper panels) and silver-stained bands (lower panels) corresponding to LacY were imaged. Experiments were carried out as described in Materials and Methods and in the legend to Fig. 1.