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. 2011 Mar 15;9(3):e1001028. doi: 10.1371/journal.pbio.1001028

Figure 4. Regional disparity in pattern behavior upon suppression of BMP signal transduction.

Figure 4

(A) Simulated pattern outcomes upon reduction of Inhibitor potency. Transition from production of circular foci to a striped pattern occurs, first on the less sensitive (simulated body) region, followed by stripe formation on the more sensitive domain (simulated neck) at higher levels of signal suppression. (B) Quantification of pattern characteristics from simulation of diminished Inhibitor potency. The proportion of total Activator positive area that is represented by circular foci is plotted. (C) β-catenin in situ hybridization detecting placode pattern upon suppression of BMP signal transduction in cultured E7.0 chicken skin. SB, p38 MAPK inhibitor SB203580; DM, Smad1/5/8 inhibitor dorsomorphin. Inhibition of p38 MAPK has little effect on the placode pattern, but yielded a robust effect in concert with suppression of Smad function. At low doses of DM stripes begin to form first on the body, then at higher doses on the neck. High doses cause β-catenin expression throughout the skin. (D) Quantification of the proportion of total β-catenin positive area that is represented by circular placodes in cultured skin treated with BMP inhibitors. Statistically significant p values are indicated above data points.