Table III. Escherichia coli strains and plasmids.

Strain or plasmid	Genotype or markers	Reference
K37	galK, rpsL	Miller and Friedman (1980)
JC10240	Hfr(PO45) thr-3000 recA56 srl-300::Tn10 relA ilv-318 spoT1 thi-1 rpsE2300	Csonka and Clark (1980)
PAL421Tr	fmsΔ1, galK, rpsL, recA56, srl-300::Tn10	Meinnel and Blanquet (1994)
PAL314Tr	mapΔ1, galK, rpsL, recA56, srl-300::Tn10	this work
JM101Tr	supE, thi, Δ(lac-pro), recA56, srl-300::Tn10, F′(traD36, proA+B+, lacIq, lacZΔM15)	Hirel et al. (1988)
pBluescriptSK	bla	Stratagene
pBEBNB	derivative of pBluescript SK with the BglII and NcoI sites inserted between the EcoRI and BamHI sites	Meinnel et al. (1993a)
pQE60	bla, C-terminal His6-tag fusion vector	Qiagen
pMAK705	cat, thermosensitive pSC101 replicon	Hamilton et al. (1989)
pUCdef	derivative of pUC18 with a chromosomal fragment from E.coli, fms, fmt′	Meinnel and Blanquet (1993)
pMGDA-GFP	derivative of pSmRS-GFP with a SalII–NcoI fragment carrying the complete sequence of chloroplast membrane lipid MGDA synthetase from spinach fused in-frame to GFP	personal gift of E.Maréchal (Grenoble)
pXL1071	bla, map	Mayaux et al. (1987)
pXΔ	bla, mapΔ1	this work
pM1A	derivative of pBluescriptSK with the full-length cDNA encoding A.thaliana MAP1A	this work
pM1B	derivative of pBluescriptSK with the full-length cDNA encoding A.thaliana MAP1B	this work
pM1C	derivative of pBluescriptSK with the full-length cDNA encoding A.thaliana MAP1C	this work
pM1D	derivative of pBEBNB with the full-length cDNA encoding A.thaliana MAP1D	this work
pM2	derivative of pBluescriptSK with the full-length cDNA encoding A.thaliana MAP2	this work
pD1a	derivative of pBluescriptSK with the full-length cDNA encoding A.thaliana PDF1A	this work
pDLe1b	derivative of pBEBNB with the full-length cDNA encoding L.esculentum PDF1B	this work
pDAt1b	derivative of pBEBNB with the full-length cDNA encoding A.thaliana PDF1B	this work
pHuPD	derivative of pBluescriptSK with the full-length cDNA encoding the H.sapiens PDF homologue	this work
pMap	derivative of pMAK705, map	this work
pQdef1a	derivative of pQE60 and pD1a with an NcoI–BamHI insertion containing the whole A.thaliana PDF1A ORF (starting at third ATG) cloned between the NcoI and BglIII sites in-frame with the 6-His tag	this work
pQdef1b	derivative of pQE60 with an NcoI–BglIII insertion containing the whole L.esculentum PDF1B cDNA and the 3′ untranslated sequence cloned between the NcoI and BamHI sites	this work
pQdef1aΔN	same as pQdef1a but lacking codons 2–78 of the def gene	this work
pQdef1bΔN	same as pQdef1b but lacking codons 2–83 of the def gene	this work
pSmGFP	bla, CamV35S-smGFP-NosT	Davis and Vierstra (1998)
pSmRSGFP	bla, CamV35S-smRSGFP(S65T)-NosT	Davis and Vierstra (1998)
pDG1a	derivative of pSmGFP with an XbaI–BamHI fragment carrying the complete 5′ sequence of A.thaliana PDF1A (up to codon I83) inserted in-frame in between XbaI and BamHI	this work
pDG1aΔ1	same as pDG1a1a but deletion of the first 18 bases (including ATG1)	this work
pDG1aΔ2	same as pDG1a1a but deletion of the first 39 bases (including ATG1 and ATG2)	this work
pDRG1a	same as pDG1a, but derivative of pSmRS-GFP	this work
pDRG1aΔ1	same as pDG1aΔ1, but derivative of pSmRS-GFP	this work
pDRG1aΔ2	same as pDRG1aΔ2, but derivative of pSmRS-GFP	this work
pDG1b	derivative of pSmGFP with an XbaI–BamHI fragment carrying the complete 5′ sequence of A.thaliana PDF1B (up to codon I82) inserted in-frame in between XbaI and BamHI	this work
pDG2b	derivative of pSmGFP with an XbaI–BamHI fragment carrying the complete 5′ sequence of L.esculentum PDF1B (up to codon I89) inserted in-frame in between XbaI and BamHI	this work
pDRG1b	same as pDG1b, but derivative of pSmRS-GFP	this work
pMG1a	derivative of pSmGFP with an XbaI–BamHI fragment carrying the 5′ sequence of A.thaliana MAP1A (up to codon I141) inserted in-frame in between XbaI and BamHI	this work
pMG1b	derivative of pSmGFP with an XbaI–BamHI fragment carrying the 5′ sequence of A.thaliana MAP1A (up to codon I126) inserted in-frame in between XbaI and BamHI	this work
pMG1c	derivative of pSmGFP with an XbaI–BamHI fragment carrying the 5′ sequence of A.thaliana MAP1A (up to codon I99) inserted in-frame in between XbaI and BamHI	this work
pMG1d	derivative of pSmGFP with an XbaI–BamHI fragment carrying the 5′ sequence of A.thaliana MAP1A (up to codon L105) inserted in-frame in between XbaI and BamHI	this work
pMG2	derivative of pSmGFP with an XbaI–BamHI fragment carrying the 5′ sequence of A.thaliana MAP2A (up to codon I126) inserted in-frame in between XbaI and BamHI	this work
pMRG2	same as pMG2, but derivative of pSmRS-GFP	this work