Figure 3. Enhancement in the level of phospho-Thr²⁸⁶ of α-CaMKII in the presence of GST-NR2B (S1303A) in the CaMKII/phosphatase coupled system.

CaMKII and PP1 were maintained simultaneously active in the reaction for 5 min and the sample was then analysed by western blotting. Panel A shows the Western blot probed using anti-phospho-Thr²⁸⁶-α-CaMKII antibody and GST fusion protein bands stained by Ponceau S. Values obtained by densitometry from four experiments were used for calculating percentage dephosphorylation shown as bar graphs in panel B. In each set, the band intensity of the sample that was not treated with PP1 [PP1 (−)] shown in A was taken as 100%. (*p value<0.05).