Table 1. ITC data for ATPγS binding to CaMKII.
Protein substrate | N | Ka (M−1) | ΔH (cal/mol) | ΔS (cal/mol*K) | ΔG (cal/mol) |
GST-NR2A | 1.28±0.0065 | 2.22×106±2.7×105 | −6814±59.15 | 5.8 | −8499 |
GST-NR2B | 1.04±0.0021 | 2.51×107±4.3×106 | −7439±32.36 | 8.48 | −9909 |
Titrations were done by adding ATPγS to CaMKII that has already been equilibrated with either GST-NR2A or GST-NR2B. Chi square values for the titrations in the presence of GST-NR2A and GST-NR2B were 2.25×104 and 8679 respectively.