Skip to main content
. 2011 Mar 15;6(3):e16495. doi: 10.1371/journal.pone.0016495

Table 1. ITC data for ATPγS binding to CaMKII.

Protein substrate N Ka (M−1) ΔH (cal/mol) ΔS (cal/mol*K) ΔG (cal/mol)
GST-NR2A 1.28±0.0065 2.22×106±2.7×105 −6814±59.15 5.8 −8499
GST-NR2B 1.04±0.0021 2.51×107±4.3×106 −7439±32.36 8.48 −9909

Titrations were done by adding ATPγS to CaMKII that has already been equilibrated with either GST-NR2A or GST-NR2B. Chi square values for the titrations in the presence of GST-NR2A and GST-NR2B were 2.25×104 and 8679 respectively.