Fig. 2. Overexpression of C/EBPγ affects globin gene switching. Mice harbouring the 70 kb human β-globin locus (line 72) were crossed with C/EBPγΔB line 1 and C/EBPγ line 21, heterozygous for the EBP transgene. RNA was isolated from E12.5 and E13.5 livers and 1 µg was used for quantitative S1 nuclease analysis of human globin gene expression (Wijgerde et al., 1996). Arrows indicate human γ-globin (h-γ) and β-globin (h-β) signals. The average of the h-γ/h-β signals with standard deviations (s.d.) and the number of fetuses analysed (n) are shown below the lanes.