Fig. 3. Fatal anaemia caused by high level expression of the EBP transgenes. (A) Wild-type (left) and C/EBPγΔB-overexpressing line 22 (right) E12.5 littermates. Note the pallor of the transgenic fetus, which has an otherwise normal gross morphological appearance. (B) Fetal livers dissected from the fetuses shown in (A). The transgenic liver (right) is small and pale relative to the control liver (left), indicative of hampered erythropoiesis. (C) Cytospins of peripheral blood and fetal liver single cell suspensions prepared from fetuses with the indicated genotype. ntg, non-transgenic; het, heterozygous; hom, homozygous. Data shown are obtained with C/EBPγΔB line 1 and C/EBPγ line 21. The slides were stained with a combined histological and neutral benzidine stain.