Table I. EBP transgenic mice generated for this study.
| Founder | Copy number | Remarks | γ-globin expression |
|---|---|---|---|
| PEV-C/EBPγ | |||
| 7 | (10) | no transmission | n.a. |
| 8a | 6 | homozygous alive | γ up in het. and hom. |
| 13 | 4 | transmission | n.d. |
| 14 | (42) | no transmission | n.a. |
| 19 | 26 | homozygous lethal | γ up in het. |
| 21a | 20 | homozygous lethal | γ up in het. |
| 22 | (22) | no transmission | n.a. |
| 28 | 12 | homozygous alive | n.d. |
| pEV-C/EBPγΔB | |||
| 1a | 22 | homozygous lethal | γ normal in het. |
| 2 | (16) | no transmission | n.a. |
| 10 | (20) | no transmission | n.a. |
| 12 | 6 | homozygous alive | γ normal in het. and hom. |
| 19 | 5 | homozygous alive | γ normal in het. and hom. |
| 21 | (20) | no transmission | n.a. |
| 22a | 40 | heterozygous lethal | n.d. |
| 14 | (< 1) | no transmission | n.a. |
n.a., not applicable; n.d., not determined; het., heterozygous; hom., homozygous for the EBP transgene. Expression of γ-globin refers to γ mRNA levels in E12.5 fetal liver in crosses with mice carrying the human β-globin locus (line 72; Strouboulis et al., 1992). There was no significant difference in the upregulation of γ-globin between the different C/EBPγ transgenics.
aData obtained with these lines are shown in the figures.