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. 2011 Mar 15;6(3):e16828. doi: 10.1371/journal.pone.0016828

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Takayama et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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BAG3 inhibits apoptosis caused by αB-crystallin mutant in C2C12 cells. αB-crystallin wild type or R120G was expressed with or without Flag-tagged BAG3 in C2C12 myoblast cells, and cells were cultured with differentiation media to induce myotube differentiation. After fixing cells, αB-crystallin and cell nuclei were stained with anti-αB-crystallin antibody and Dapi solution, respectively. Cells with nuclear fragmentation typical of apoptosis were counted and statistically analyzed.