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Oligosaccharides are attached to the array using different spacer arms. Sp0: -CH2CH2NH2; Sp8: -CH2CH2CH2NH2; Sp10: -NHCOCH2NH; Sp21: -N(CH3)-O-(CH2)2-NH2. RFU: Relative Fluorescence Units. Yellow circles, galactose; blue circles, glucose; yellow squares, N-Acetyl-galactosamine; blue squares, N-Acetyl-glucosamine; red triangles, fucose; pink diamonds, sialic acid. ND: Not detected.
