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. 2011 Mar 15;6(3):e16890. doi: 10.1371/journal.pone.0016890

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Su et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Image of a dye label (Alexa-546) on a DNA backbone (backbone not shown) with ms exposure time. (B) 2D surface plot of the raw image (intensity of the dye vs. the X Y coordinates). (C) Image of one T4 DNA fragment (36 microns) with backbone (red) and internal labels (green). (D) Time series (8 seconds) of the DNA showing the fluctuations of backbone and internal labels. In (D), the red trace is the backbone and the green traces are the trajectories of internal dye labels.

Inline graphic — (A) Image of a dye label (Alexa-546) on a DNA backbone (backbone not shown) with ms exposure time. (B) 2D surface plot of the raw image (intensity of the dye vs. the X Y coordinates). (C) Image of one T4 DNA fragment (36 microns) with backbone (red) and internal labels (green). (D) Time series (8 seconds) of the DNA showing the fluctuations of backbone and internal labels. In (D), the red trace is the backbone and the green traces are the trajectories of internal dye labels.