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. 2001 Jan;12(1):239–250. doi: 10.1091/mbc.12.1.239

Figure 1.

Figure 1

SDS-PAGE of bacterially expressed, purified wild-type, and mutant rat caldesmon. Both wild-type and mutant caldesmon were expressed in bacteria, and purified by DEAE-cellulose followed by calmodulin-Sepharose column. Both wild-type and mutant caldesmon showed >95% purity. Lane A, wild-type caldesmon; lane B, 7th mutant caldesmon; lane C, molecular mass markers (indicated in kDa at the right of the figure). (D) Actin binding of wild-type and 7th mutant caldesmon. ○, wild-type caldesmon; ●, 7th mutant caldesmon.