Table 1.

Inhibition of cell division of Xenopus embryos by 7th mutant

	Volume	Estimated final conc.*	No. of injection	Blockage	Delay	Normal
	nl	μM		%
Buffer	50	0	42	0	2	98
Wild	50	16.5	64	5	29	66
Wild	25	8.25	41	2	37	61
7th	50	16.5	60	63	37	0
7th	25	8.25	22	14	82	5

^* The needle concentration of caldesmon is 165 μM. The calculation is based on an estimate that the volume of one blastomere at the two-cell stage of Xenopus embryos is 0.5 μl.