Table 2.
Cytokine secretion by PMA-differentiated U937 macrophages following stimulation with S. suis P1/7 and its SspA deficient mutant G6G.
| Strain | Amount secreted of cytokines (pg/ml) | ||||
|---|---|---|---|---|---|
| IL-1β | TNF-α | IL-6 | CXCL8 | CCL5 | |
| Control | 51 ± 3 | 217 ± 2 | 10 ± 1 | 5245 ± 432 | 2116 ± 4 |
| S. suis P1/7 | 161 ± 8 | 1800 ± 11 | 1160 ± 21 | 611000 ± 756 | 13355 ± 564 |
| S. suis G6G | 120 ± 3* | 1030 ± 14* | 690 ± 6* | 653000 ± 634* | 15664 ± 34* |
The data are the means ± SD of triplicate assays for three separate experiments. Asterisks indicate a significant difference in cytokine secretion by macrophages stimulated with the SspA deficient mutant (G6G) in comparison with the parental strain at P < 0.01.