Figure 4. cold is not required for establishment of epithelial cell polarity.

(A–D) Single confocal sections showing the overall morphology of either wild type embryos or mutant combinations lacking the cold maternal contribution (cold M), the zygotic one (cold Z) or both (cold M & Z), all stained for Dlg. (E–I′) Subcellular distribution of FasIII and Dlg in trachea, salivary gland and hindgut of stage 16 embryos of the indicated genotypes. Notice FasIII uniform distribution in the lateral membrane of the mutant tissues (E–I, arrows). A portion of Dlg (E′–I′) was redirected to the lateral membrane in the mutant tissues (arrows), although apical accumulation was still visible in the salivary gland and hindgut cells (open arrowheads).