Figure 7. The cold gene is autonomously required in the wing disc for SJ organisation.

(A–C′) Confocal pictures of third larval instar wing imaginal discs containing M⁺ cold^f02290 clones generated in first instar larvae and stained as indicated. The homozygous cold mutant cells lacking GFP (green) can be distinguished from the surrounding GFP positive heterozygous tissue. A–C correspond to optical planar x-y sections taken at the level of the SJ and A′–C′ show optical z-sections of the same discs. The planar views revealed abnormally low levels of FasIII and Dlg (red in left panels, greyscale in right panels) in the apical part of the cold mutant cells (arrows). The z planes show that Dlg was found accumulating in a wider apical domain (open arrowhead), whereas FasIII was distributed uniformly along the lateral part of the cells (arrow). A normal Crb apical distribution (red in left panel, greyscale in the right panel) was observed in cold mutant cells.