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. 2011 Mar 15;6(3):e17763. doi: 10.1371/journal.pone.0017763

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Hijazi et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A–C′) Confocal single sections of salivary glands belonging to third instar larvae carrying the UASFLAG-cold construct and the tubulinGAL80^ts repressor. (A,A′) At 25°C and in presence of the bouGAL4 driver, FLAG-Cold (cyan in upper panels, greyscale in lower panels) was present at high levels in the salivary gland lumen and the cell body. (B,B′) After 40 hours at 18°C the cell body staining disappeared but FLAG-Cold persisted in the salivary gland lumen (open arrowhead) and in lateral cell regions (arrows) accumulating FasIII (seen in red). A nuclear staining was also observed but it corresponds to a unspecific interaction of the FLAG antibody, as it was also present in larvae not carrying the bouGAL4 driver (C,C′).