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Immunocytochemical Analysis of MAFbx/atrogin-1. DAB-mediated immunocytochemistry was used to examine the protein abundance of MAFbx/atrogin-1 in uncompressed and compressed muscles of DMSO- and MG132-treated animals. Uncompressed muscle of both DMSO- and MG132-treated rats did not show any immunoreactivity of MAFbx/atrogin-1. Apparent positive immunoreactivity was detected in the muscle fibres of compressed muscle in only DMSO-treated rats but not in MG132-treated rats.
