Table 3.
Gene name, origin, accession numbers, and functional annotation of spruce TPS
Gene | Clone ID (genotype) | Functional Annotation* | NCBI Accession |
---|---|---|---|
MONOTERPENE SYNTHASES | |||
Pg×eTPS-Car1 | WS0063_F08 (Fa1-1028) | (+)-3-Carene synthase | HQ426152 |
PsTPS-Car1 | WS02910_I02 (FB3-425) | (+)-3-Carene synthase | HQ426167 |
PgTPS-Cin | WS02628_N22 (PG29) | 1,8-Cineole synthase | HQ426160 |
PgxeTPS-Cin | WS00921_D15 (Fa1-1028) | 1,8-Cineole synthase | HQ426156 |
PsTPS-Cin | WS0291_H24 (FB3-425) | 1,8-Cineole synthase | HQ426165 |
PgTPS-Lin | WS0054_P01 (PG29) | (-)-Linalool synthase | HQ426151 |
PsTPS-Lin-1 | WS0285_L07 (FB3-425) | (-)-Linalool synthase | HQ426164 |
PsTPS-Lin-2 | WS02915_K02 (FB3-425) | (-)-Linalool synthase | HQ426168 |
PsTPS-Phel-1 | WS02729_A23 (FB3-425) | (-)-β-Phellandrene synthase | HQ426162 |
PsTPS-Phel-2 | WS0296_I22 (FB3-425) | (-)-β-Phellandrene synthase | HQ426169 |
PsTPS-Phel-3 | WS0276_M12 (FB3-425) | (-)-β-Phellandrene synthase | HQ426163 |
PsTPS-Phel-4 | WS01042_E12 (Gb2-229) | (-)-β-Phellandrene synthase | HQ426159 |
PgTPS-Pin-1 | WS00725_G07c1 (PG29) | (-)-α/β-Pinene synthase | HQ426153 |
PgTPS-Pin-2 | WS00725_G07c2 (PG29) | (-)-α/β-Pinene synthase | HQ426154 |
PsTPS-Pin | WS0291_K15 (FB3-425) | (-)-α/β-Pinene synthase | HQ426166 |
SESQUITERPENE SYNTHASES | |||
Pg×eTPS-Far/Oci | WS00926_E08 (Fa1-1028) | (E,E)-α-Farnesene/(E)-β-ocimene synthase | HQ426157 |
PgTPS-Hum | WS0074_O16 (PG29) | α-Humulene synthase | HQ426155 |
Pg×eTPS-Lonf | WS00927_M20 (Fa1-1028) | Longifolene synthase | HQ426158 |
PsTPS-Lonp | WS02712_A08 (FB3-425) | α-Longipinene synthase | HQ426161 |
DITERPENE SYNTHASES | |||
PsTPS-Iso | pSW06061903 (Haney 898) | Isopimaradiene synthase | HQ426150 |
PsTPS-LAS | WS0299_C21 (FB3-425) | Levopimaradiene/abieta-diene synthase | HQ426170 |
*Functional annotation is based on the main terpenoid product(s) of recombinant enzymes expressed in E. coli. Most TPSs produced multiple products, as shown in Table 2.