Table 2.

HBV genotyping primers sequence

Primer Sequence	a(position, specificity, and polarity)
First PCR
P1 5'-TCA CCA TAT TCT TGG GAA CAA GA-3'	(nt 2823-2845, universal, sense)
S1-2 5'-CGA ACC ACT GAA CAA ATG GC-3'	(nt 685-704, universal, antisense)
Second PCR
Mix A
B2 5'-GGC TCM AGT TCM GGA ACA GT-3'	(nt 67-86, types A to E specific, sense)
BA1R 5'-CTC GCG GAG ATT GAC GAG ATG T-3'	(nt 113-134, type A specific, antisense)
B1R 5'-CAG GTT GGT GAG TGA CTG GAG A-3'	(nt 324-345, type B specific, antisense)
BC1R 5'-GGT CCT AGG AAT CCT GAT GTT G-3'	(nt 165-186, type C specific, antisense)
Mix B
BD1 5'-GCC AAC AAG GTA GGA GCT-3'	(nt 2979-2996, type D specific, sense)
BE1 5'-CAC CAG AAA TCC AGA TTG GGA CCA-3'	(nt 2955-2978, type E specific, sense)
BF1 5'-GYT ACG GTC CAG GGT TAC CA-3'	(nt 3032-3051, type F specific, sense)
B2R 5'-GGA GGC GGA TYT GCT GGC AA-3'	(nt 3078-3097, types D to F specific, antisense)

a An "M" represents a nucleotide that could be either an A or a C; a"Y" represents a nucleotide that could be either a C or a T. nt: nucleotide.