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. 2010 Oct 13;30(41):13838–13849. doi: 10.1523/JNEUROSCI.3846-10.2010

Figure 7.

Figure 7.

Ubiquitous overexpression of Slit2-GFP causes astray-like phenotypes in the developing, but not the regenerating optic projection. A, B, Lateral views (maximal-intensity projections) of optic axons in heat-shocked embryos at 48 hpf show an essentially wild-type projection in hsp70l:mcherry control embryos, but in hsp70l:slit2-GFP embryos the tract is severely disorganized with anterior (arrow) and posterior (arrowhead) misprojections. C–F, After repeated heat-shocks the entire brain of hsp70l:slit2-GFP animals (F) shows intense GFP fluorescence, compared with non-heat-shocked controls (E). Similarly, immunodetection of GFP in sections of the telencephalon shows homogeneous immunoreactivity after heat shock (D), but not in non-heat-shocked controls (C). G–L, Regenerating optic axons in heat-shocked wild-type (wt) and hsp70l:slit2-GFP transgenic fish do not grow into the telencephalon (G, H) or the posterior commissure (PC in K, L) and exclusively populate the contralateral tectum (arrows in I, J). Scale bars: A, B, 50 μm; C, D, 200 μm; E, F, 1 mm; G, H, 200 μm; I, J, 100 μm; K, L, 50 μm.