Fig. 7. Indirect immunofluorescence microscopy to confirm that plasma membrane localization of the Sso2Q228R protein is indistinguishable from that of wild-type Sso2p. Yeast transformants of strain Δ4-2D containing different pairs of either wild-type (WT Q/R) or mutant (Mut R/R; Mut R/Q; Mut Q/Q) SSO2 and SNC2 alleles from centromeric plasmids (indicated in Figure 2) were fixed and prepared for immunocytochemistry. HA and c-Myc epitope-specific anibodies were used to detect the epitope-tagged SNARE proteins. Cy2-conjugated antibodies track Sso2wt or Sso2Q228R proteins (shown in green), while Cy3-conjugated antibodies labeled Snc2wt or Snc2R52Q proteins (shown in red). DAPI staining of DNA was used to localize the nucleus (blue).