(a–c) The apparent temporal diffusion spectrum depends on intra-nucleus diffusion coefficient (Dnuc) and T2. Four Dnuc were used in the simulation, i.e. 0.5, 1.0, 1.31 and 1.82 μm2/ms. (d–f) ADCs at some frequencies (100, 500 and 1000Hz) were plotted against Dnuc. For comparison, ADCs obtained by the PGSE method were also provided.