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. 2011 Mar;162(6):1261–1273. doi: 10.1111/j.1476-5381.2010.01154.x

Figure 3.

Figure 3

Effects of tomato cystine-knot miniproteins on HUVEC viability and proliferation. (A) WST-8 (MTT) cell proliferation assay. Number of live cells measured after 24 and 48 h of treatment is shown (percentage of control value, 3 independent experiments). Statistical analysis was performed using one-way anova and Bonferroni's tests. (B) Annexin V-propidium iodide assay. The percentage of viable cells after 48 h of treatment with or without 200 nM TCMP-2 is presented (n= 3). Student's t-test was applied. (C) Number of HUVEC per well counted 24, 48, 72, 96 h after treatment with or without 170 nM TCMP-2 (n= 3). The two-way anova for repeated measures was applied. (D) EdU proliferation assay. The percentage of proliferating cells after 48 h of treatment with or without 200 nM TCMP-2 is presented (n= 3). Student's t test was applied. (E) Quantitative analysis of cell migration 12 h after treatment with 100 nM TCMP-2, AG1478 500 nM or no added compounds (n= 3–4 independent experiments, three replicates). The one-way anova and Bonferroni's tests were applied. Data presented in all panels as means ± SD. ***P < 0.001 versus controls. Ctrl, control; rT1, purified recombinant TCMP-1; rT2, purified recombinant TCMP-2; fruit, TCMP miniproteins purified from tomato fruit; T1 N-term and T1 C-term indicate the treatment with peptides corresponding to the N-terminal (17 amino acids in length) or the C-terminal (20 amino acids in length) regions of TCMP-1 respectively.