Figure 3.

Internalization of P2Y₁ receptors in Madin-Darby canine kidney (MDCK) cells is dynamin- and clathrin-dependent. MDCK cells stably expressing recombinant P2Y₁ receptors were incubated with 10 µM 2MeSADP for 30 min in the absence (Buffer) or presence of monodansylcadaverine (MDC; an inhibitor of clathrin endocytosis) or dynasore (an inhibitor of the dynamin GTPase). MRS2500-binding sites were then quantified. All values are normalized to the total amount of radiolabelled MRS2500 binding in the absence of agonist treatment. Data shown are means of triplicate samples ± SEM from a representative experiment (n= 3). **P < 0.01.