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. 2011 Mar;162(6):1351–1363. doi: 10.1111/j.1476-5381.2010.01141.x

Figure 6.

Figure 6

Nicotine and ethanol modulate miniature excitatory postsynaptic current (mEPSC) activity. A. The upper traces (A1) show raw control (Con) and during nicotine application (Nic) on mEPSC responses after abolishing GABAergic transmission with bicuculline methiodide (BMI) and CGP 52432, and tetrodotoxin (0.5 µM) to block action potential induced spontaneous EPSCs. (A2) Traces of the averaged mEPSCs before (Con) and during nicotine (Nic) treatment. (B1) These traces show the effects of ethanol (EtOH) or ethanol plus nicotine application on mEPSCs, and the averaged waveforms for each condition (B2). The summary data for nicotine and ethanol effects on mEPSC amplitude (C) and frequency (D) are shown. These data indicate that ethanol inhibits nicotine-enhanced both amplitude and frequency of mEPSCs. Number of cells recorded in C and D: Con (12), EtOH (7), Nic (9), EtOH + Nic (6). Scale bars – in A1 and B1: 10 pA, 2.0 s; in A2: 2 pA, 4 ms; B2: 4 pA, 4 ms. *P < 0.05; **P < 0.01; ***P < 0.001.