Figure 6.

Effects of baicalein treatment on phosphorylation of ERK1/2 and CREB after high-frequency stimulation (HFS) in rat hippocampal CA1 region. (A) Representative images of immunoblots using antibodies against phosphorylated ERK1/2 (pERK1/2), ERK1/2 and β-actin. HFS stimulation induced an activation of ERK1/2 phosphorylation 5 min after HFS. Pre-incubation of hippocampal slices with baicalein (1 µM) did not affect ERK1/2 phosphorylation 5 min after HFS. (B) Summary data of phosphorylated ERK1/2 and total ERK1/2 expression by densitometry. Data were expressed as percentage of value of controls without stimulation. #P < 0.05 versus control, n= 4. (C) Representative images of immunoblots using antibodies against phosphorylated CREB (Ser¹³³), total CREB and β-actin. HFS stimulation induced an activation of CREB phosphorylation 5 min after HFS. Pre-incubation of hippocampal slices with baicalein (1 µM) further increased CREB phosphorylation 5 min after HFS. (D) Summary data of phosphorylated CREB and total CREB expression by densitometry. Data were expressed as percentage of value of controls without stimulation. #P < 0.05 versus control, *P < 0.05 versus HFS, n= 4.