Immunohistochemistry for proliferating cell nuclear antigen (PCNA) in the study groups. (A–C) Representative pictures stained by immunohistochemistry for PCNA of a LETO rat (A), an OLETF-CON rat (B) and an OLETF-SIL rat (C). Original magnifications, ×200. (D, E) Quantitative score of immunohistochemical analysis for PCNA in glomerular cells (D) and tubulointerstitial cells (E) in the study groups. Data are expressed as means ± SEM. Mann–Whitney test: *P < 0.01, **P < 0.001: LETO vs. OLETF-CON; #P < 0.001, ##P < 0.0001: OLETF-SIL vs. OLETF-CON. LETO, Long-Evans Tokushima Otsuka; OLETF, Otsuka Long-Evans Tokushima Fatty.