Figure 3.

DNA fragmentation and chromatin condensation in LiCl-treated macrophages. (A) Quantification of apoptotic cells according to the Vindelov method. J774A.1 macrophages were treated with 30 mM LiCl or 30 mM NaCl for 0–24 h. DNA histograms of LiCl- and NaCl-treated macrophages, as well as untreated cells, are shown. Fragmented DNA appears in a subG₁-peak on DNA histograms (PI-A, area of PI-positive cells). Data represent mean ± SEM of three independent experiments. *P < 0.05 versus control (anova, followed by Dunnett test). Evaluation of chromatin condensation (white arrowheads) and necrotic cells (red arrowheads) after staining with Hoechst 33342 and PI in J774A.1 macrophages treated with 30 mM LiCl for 0 h (B), 4 h (C) and 24 h (D).