Figure 4.
Lithium-sensitive inositol monophosphatase (IMPase) (IMPA1) is inhibited by LiCl in macrophages. (A) Levels of IP3 in J774A.1 macrophages and smooth muscle cells (SMCs) treated with 30 mM LiCl for 0–16 h. Results represent mean ± SEM of three independent experiments. *P < 0.05 versus 0 h (control) (anova, followed by Dunnett test). (B) Western blot analysis of IMPA1 and caspase-3 cleavage in J774A.1 macrophages transfected with siControl or siRNA IMPA1 for 0–3 days. (C) Viability of J774A.1 macrophages and mouse SMCs transfected with siControl or siRNA specific for IMPA1 at day 3. Bar graphs represent mean ± SEM of four independent experiments. **P < 0.01 versus siControl (paired-samples t-test).