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. 2011 Mar;162(6):1410–1423. doi: 10.1111/j.1476-5381.2010.01152.x

Figure 8.

Figure 8

Terminal deoxynucleotidyl transferase dUTP nick-end labelling (TUNEL) labelling as a measure for apoptosis after local in vivo treatment of rabbit atherosclerotic carotid arteries with saline (control) or LiCl for 3 days. (A) Double staining of RAM 11 (macrophages, blue) with TUNEL (DNA fragmentation, red) I, intima; M, media; Ad, adventitia; scale bar = 50 µm. Magnification of boxed areas shows co-localization of RAM 11 with TUNEL (arrowheads), only in the intimal area of LiCl-treated atherosclerotic carotid arteries. Scale bar = 30 µm. (B) Percentage TUNEL-positive area in the intima or in the media. Results are shown as mean± SEM. *P < 0.05 versus control (n= 10, Mann–Whitney U-test). (C) Double staining of α-SMC actin (SMCs, blue) with TUNEL (DNA fragmentation, red) I, intima; M, media; Ad, adventitia. Magnification of boxed areas shows no co-localization of α-SMC actin with TUNEL.