Figure 3.

SNAP- and CLIP-tagged forms of the human OX₁ and CB₁ receptor generate functional responses. Flp-In™ T-REx™ 293 cells harbouring VSV-G-SNAP-OX₁ (A), HA-CLIP-CB₁ (B), were untreated (open symbols) or treated with doxycycline (filled symbols) to induce expression of the relevant receptor construct. Varying concentrations of orexin A (A) or CP55940 (B) were added and either alteration in [Ca²⁺]_i (A) or phosphorylation of the ERK1/2 MAP kinases (B) assessed. Data are combined from 5 independent experiments and represent means ± SEM.