Figure 8.

CLIP-Lumi4Tb does not provide an improved means to detect expression and regulation of HA-CLIP-CB₁. (A) Cells harbouring HA-CLIP-CB₁ were induced with varying concentrations of doxycycline. Receptor was measured using CLIP-Lumi4-Tb emission, [³H]-SR141716A binding and anti-SNAP/CLIP binding. Data are shown as signal to background ratios. Comparisons of the signal to background ratios for each approach following treatment with 10 ng·mL⁻¹ doxycycline are shown in the bottom right panel. (B) Cells induced to express HA-CLIP-CB₁ were treated with varying concentrations of CP55940, rimonabant or O2050 for 60 min. CLIP-Lumi4-Tb was added and emission at 620 nm measured. Data represent means ± SEM from at least four independent experiments, **P < 0.01.