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. Author manuscript; available in PMC: 2011 Mar 16.
Published in final edited form as: Glycobiology. 2009 Dec 18;20(4):461–472. doi: 10.1093/glycob/cwp198

Figure 6. SPR sensorgrams of scFv 69-4 against immobilized 2.4/2.4PS4P neoglycoconjugate.

Figure 6

Sensorgrams were recorded for protein concentrations ranging from 0.01 μM to 1.4 μM (A). Results of a standard steady state analysis using the Biacore T100 Evaluation Software v. 1.1 at report points 4 s before stop of analyte injection and with a window of 5 s (B). The Rmax and KD values predicted from the fitting routine are given. The scFv was purified by gel filtration immediately prior to the analysis.