Figure 8. Detection of chlamydial inclusions in tissue culture with monoclonal antibodies S5-10, S69-4, Fab of S45-18 and NH2240-31.
Tissue culture monolayers were infected with C. psittaci, C. pneumoniae or C. trachomatis, and after fixation incubated with the indicated concentrations of antibody or fragments thereof. Reactions with scFv were developed with a c-myc specific second antibody (clone 9E10) and a third IgG H+L binding antibody conjugated to FITC. For analyses using Fab or mAb the second antibody was omitted.