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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Nat Immunol. 2010 Nov 7;11(12):1136–1142. doi: 10.1038/ni.1960

Figure 3. Differential role of ASC in NLRC4 signalling.

Figure 3

(a) Competitive index of ST-FliCON vs. ST-WT as in Figure 2 for WT (n=6) and Asc−/−(n=4) mice. (b, c) BMDM from WT, Asc−/− or Nlrc4−/−mice were infected as in Figure 1 with ST-FliCON and IL-1β secretion or cytotoxicity were determined by ELISA and LDH release 6 hours post infection. (d) WT BMDM infected with ST-WT or ST-FliCON and pyroptosis measured by LDH release 8h post infection. (e) Cytotoxicity induced by ST-FliCON was monitored with or without 10 mM glycine added to the media and cytotoxicity determined 7h post infection. In vitro infections are representative of at least three experiments. * = p < 0.05, NS = p > 0.05 from relevant control.