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. Author manuscript; available in PMC: 2011 Dec 7.
Published in final edited form as: Mol Cancer Ther. 2010 Dec 7;9(12):3330–3341. doi: 10.1158/1535-7163.MCT-10-0442

Figure 5. DTIC partially inhibits the cell cycle progression.

Figure 5

(A and B) SK-MEL-28 (A) and SK-MEL-5 (B) cell were treated with 50 μmol/L dose of DTIC for 48 h and subjected for Flow cytometry analysis. The representative cell cycle profile is shown. Histograms on the right represent the percentage of cells in the different phases of the cell cycle form three independent experiments. (C) Damaged DNA was visualyzed by the Comet assay in SK-Mel-5, SK-Mel28 and SK-MEL-2 cells following exposure to 50 μmol/L DTIC for 48 h. Representative micrograph of fluorescent DNA stain of control cells, showing undamaged DNA remaining. whereas DTIC-treated cells showing denatured DNA fragments migrating out from cell. (D) %DNA damage was quantified and mean is represented with standered deviation as error bars.