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. Author manuscript; available in PMC: 2011 Dec 7.
Published in final edited form as: Mol Cancer Ther. 2010 Dec 7;9(12):3330–3341. doi: 10.1158/1535-7163.MCT-10-0442

Figure 6. DTIC enhances the efficacy of RRD-251.

Figure 6

(A) SK-MEL-2 cell were pretreated with 50 μmol/L of DTIC for 48 before the exposure to RRD-251 for further 4 hr. Apoptosis was detected by the appearance of decreased caspase-3 and cleavage of PARP by western blot analysis. DTICas well as RRD-251 alone resulted in apoptosis. (B and C) SK-MEL-28 (B; left panel) and SK-MEL-5 (C; left panel) cell were pretreated with 50 μmol/L of DTIC for 48 before the exposure to RRD-251 for further 4 hr. Apoptosis was detected by the appearance of cleaved caspase-3 and PARP by western blot analysis. DTIC alone did not result in apoptosis (DTIC-lane). Pretreatment of DTIC before RRD-251 addition resulted in increased apoptosis as compared to RRD-251 treatment alone (DTIC+RRD-251-lane Vs RRD-251-lane) in both the cells. (B and C; right panels) The caspase-3 activity was quantified using Caspase Glo 3/7 kit in SK-MEL-28 and SK-MEL-5 cells. The pretreatment of DTIC before RRD-251 exposure resulted in significantly higher (p value <0.05) caspase activity than these treatments alone in both the cell lines.